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Similar to OECD 301D, the OECD 302C Modified MITI Test (II) measures the DO consumption during biodegradation and the degradation percentage is calculated as the ratio of DO consumption to the theoretical oxygen demand (ThOD). This process also involves the calculation of BOD (biochemical oxygen demand).
Compared to other methods in the OECD 302 series, OECD 302C has the top levels of simplicity and applicability.
The formula of the test substance and its purity may be known to calculate the ThOD. If the ThOD cannot be obtained, the chemical oxygen demand (COD) may be experimentally determined and used as a less satisfactory alternative. At Aropha, we provide free COD analysis if this method is to be used.
This method is capable of testing samples that are highly soluble, poorly soluble, volatile, and/or adsorbing. While testing poorly soluble compounds, however, the shaking or stirring of the solutions during incubation might be helpful to improve the dissolution/dispersion.
Different testing methods are applicable for materials with different properties. Below is a summary of the applicability of OECD 302C. You can find the applicability for many other methods on Aropha Resource Center
|Test||Analytical method||Sample info required||Poorly soluble||Volatile||Adsorbing|
|302C (Modified MITI Test (II))
||Respirometry: dissolved oxygen
||ThOD or COD||+/-||+||+|
*"Sample info required" is the information needed to calculate the biodegradation percentages. This must be available for a selected method.
*"COD" analysis is available and free at Aropha.
To learn more about different types of biodegradation tests, their applicability, biodegradation mechanisms, and many other information such as case studies, publications, and blogs, please check our Aropha Resource Center