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ASTM D5864 - Biodegradability Test of Lubricant - CO2 evolution (ISO/IEC 17025:2017 Certified)

ASTM D5864 is an aqueous aerobic biodegradability test that determines the biodegradability of lubricants or their components by measuring CO2 evolution during biodegradation in continuously aerated bottles.

ASTM D5864 Key Summary

Similar to the OECD 301B CO2 Evolution Test, ASTM D5864 measures the CO2 evolution to reflect the biodegradation of fully formulated lubricants or their components, based on the exposure to activated sludge from municipal wastewater treatment plant as the inoculum under laboratory conditions.

This method is designed for all lubricants that are not volatile and are not inhibitory to the inoculum at the test concentration. It is intended to specifically address the issues associated with insoluble substances and complex mixtures that are commonly found in lubricants.

It can handle materials that are soluble, insoluble and/or absorbing. Good dispersion with continuous mixing is usually required for better performance.

Test substances that achieve high levels of biodegradation are assumed to be easily biodegradable in most aerobic aquatic environments.

Requirement and Applicability

Different testing methods are applicable for materials with different properties. Below is a summary of the applicability of ASTM D5864. Please check our Method Selection Guide to select the most appropriate method for your materials. You can also find the applicability for many other methods on Aropha Resource Center

TestAnalytical methodSample info required *Poorly solubleVolatileAdsorbing
ASTM D5864 (CO2 evolution test)
CO2 evolutionOrganic carbon content *+-+

*"Sample info required" is the information needed to calculate the biodegradation percentages. This must be available for a selected method.
*"Organic carbon content" is the ratio of the organic carbon weight to the weight of the sample. It can be calculated by the sample formula (e.g., acetic acid C2H4O2, carbon content is 12*2/(12*2+1*4+16*2)=40%). Try our Online C% Calculator. If the formula is unknown, we can send the sample out to a third party lab for you for analysis (normally $110 with a 10-day turnaround time).

Aropha Resource Center

To learn more about different types of biodegradation tests, their applicability, biodegradation mechanisms, and many other information such as case studies, publications, and blogs, please check our Aropha Resource Center

Aropha resource center


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