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The OECD 301D Closed Bottle Test measures the dissolved oxygen (DO) consumption during aerobic biodegradation in a closed bottle setup without head space. The degradation percentage is calculated as the ratio of DO consumption to the theoretical oxygen demand (ThOD).
Compared to some other methods, OECD 301D has the top levels of simplicity and applicability.
The formula of the test substance and its purity may be known to calculate the ThOD. If the ThOD cannot be obtained, the chemical oxygen demand (COD) may be experimentally determined and used as a less satisfactory alternative.
Information on the toxicity of the test substance is helpful in determining the appropriate dosage so that the material does not inhibit bacteria at the concentration tested.
This method is capable of testing samples that are highly soluble, volatile, and/or adsorbing.
Different testing methods are applicable for materials with different properties. Below is a summary of the applicability of OECD 301D. Please check our Method Selection Guide to select the most appropriate method for your materials. You can also find the applicability for many other methods on Aropha Resource Center
|Test||Analytical method||Sample info required *||Poorly soluble||Volatile||Adsorbing|
|OECD 301D (Closed bottle test)||Dissolved oxygen||ThOD or COD *||+/-||+||+|
*"Sample info required" is the information needed to calculate the biodegradation percentages. This must be available for a selected method.
*"ThOD" can be easily calculated based on the formula of the sample. Try our Online ThOD Calculator.
*"COD" can be used as an alternative to ThOD if the formula of the material is unknown. We provide COD analysis at Aropha.
To learn more about different types of biodegradation tests, their applicability, biodegradation mechanisms, and many other information such as case studies, publications, and blogs, please check our Aropha Resource Center