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The OECD 301F closed respirometer method determines the biodegradability of a material by measuring oxygen consumption during the biodegradation process in a period of 28 days in a closed bottle setup (with head space). The degradation percentage is calculated as the ratio of oxygen consumption to the theoretical oxygen demand (ThOD).
The oxygen consumption is usually determined by measuring the quantity of oxygen (produced electrolytically) required to compensate the consumed one to maintain a constant gas volume/pressure in the respirometer flask, or from the change in the volume or pressure in the flask without compensating the consumed oxygen. The evolved CO2 is absorbed by sodium hydroxide solution present in a tube in the flask.
The formula of the test substance and its purity may be known to calculate the ThOD. If the ThOD cannot be obtained, the chemical oxygen demand (COD) may be experimentally determined and used as a less satisfactory alternative.
Information on the toxicity of the test substance is helpful in determining the appropriate dosage so that the material does not inhibit bacteria at the concentration tested.
This method is capable of testing samples that are highly soluble, poorly soluble, insoluble, volatile, involatile, and/or adsorbing. It has therefore been often used for materials that the OECD 301B and OECD 301D methods cannot handle (e.g., insoluble and volatile).
OECD 301F is our first recommended method due to its simple experimental setup and high applicability.
Different testing methods are applicable for materials with different properties. Below is a summary of the applicability of OECD 301D. Please check our Method Selection Guide to select the most appropriate method for your materials. You can also find the applicability for many other methods on Aropha Resource Center
|Test||Analytical method||Sample info required *||Poorly soluble||Volatile||Adsorbing|
|OECD 301F (Closed respirometer test)||Oxygen consumption||ThOD or COD *||+||+/-||+|
*"Sample info required" is the information needed to calculate the biodegradation percentages. This must be available for a selected method.
*"ThOD" can be easily calculated based on the formula of the sample. Try our Online ThOD Calculator.
*"COD" can be used as an alternative to ThOD if the formula of the material is unknown. We provide COD analysis at Aropha.
To learn more about different types of biodegradation tests, their applicability, biodegradation mechanisms, and many other information such as case studies, publications, and blogs, please check our Aropha Resource Center