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The most import fact about ISO 16221 is that it targets marine environment, but is directly based on five ISO methods for fresh water, including ISO 7827, 10707, 10708, 9439, and 14593. The corresponding analytical methods/system setups are (I) DOC die-away, (II) closed bottle, (III) two-phase closed bottle, (IV) CO2 evolution, and (V) CO2 headspace tests. We currently provide analysis for (II) and (IV). Below is a quick review of these five methods:
▶ ISO 7827 - Water quality — Evaluation of the "ready", "ultimate" aerobic biodegradability of organic compounds in an aqueous medium — Method by analysis of dissolved organic carbon (DOC).
▶ ISO 10707 - Water quality — Evaluation in an aqueous medium of the "ultimate" aerobic biodegradability of organic compounds — Method by analysis of biochemical oxygen demand (closed bottle test).
▶ ISO 10708 - Water quality — Evaluation in an aqueous medium of the ultimate aerobic biodegradability of organic compounds — Determination of biochemical oxygen demand in a two-phase closed bottle test.
▶ ISO 9439 - Water quality — Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium — Carbon dioxide evolution test.
▶ ISO 14593 - Water quality — Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium — Method by analysis of inorganic carbon in sealed vessels (CO2 headspace test).
Information on the toxicity of the test substance is helpful in determining the appropriate dosage so that the material does not inhibit bacteria at the concentration tested.
Depending on the specific method to be selected, this test may handle materials that are insoluble, volatile, and/or adsorbing.
Different testing methods are applicable for materials with different properties. Below is a summary of the applicability of ISO 16221. Please check our Method Selection Guide to select the most appropriate method for your materials. You can also find the applicability for many other methods on Aropha Resource Center
|Test||Analytical method||Sample info required *||Poorly soluble||Volatile||Adsorbing|
|(I) DOC die-away (based on ISO 7827)||DOC
||Organic carbon content*
|(II) Closed bottle test (based on ISO 10707)
||ThOD or COD*
|(III) Two-phase closed bottle test (based on ISO 10708)
||ThOD or COD
|(IV) CO2 evolution test (based on ISO 9439)
||CO2 evolution||Organic carbon content||+||-||+|
|(V) CO2 head space test (based on ISO 14593)
||CO2 evolution||Organic carbon content||+||+||+|
*"Sample info required" is the information needed to calculate the biodegradation percentages. This must be available for a selected method.
*"Organic carbon content" is the ratio of the organic carbon weight to the weight of the sample. It can be calculated by the sample formula (e.g., acetic acid C2H4O2, carbon content is 12*2/(12*2+1*4+16*2)=40%). Try our Online C% Calculator. If the formula is unknown, we can send the sample out to a third party lab for you for analysis (normally $110 with a 10-day turnaround time).
*"ThOD" can be easily calculated based on the formula of the sample. Try our Online ThOD Calculator.
*"COD" can be used as an alternative to ThOD if the formula of the material is unknown. We provide COD analysis at Aropha.
To learn more about different types of biodegradation tests, their applicability, biodegradation mechanisms, and many other information such as case studies, publications, and blogs, please check our Aropha Resource Center