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ISO 10707: Ultimate Biodegradability Test - Closed Bottle (ISO/IEC 17025:2017 Certified)

ISO 10707 is an aqueous aerobic biodegradability test that determines the ultimate aerobic biodegradability of organic compounds in aqueous medium by measuring dissolved oxygen consumption during biodegradation in closed bottles. The test normally lasts for 28 days.


Similar to OECD 301D, ISO 10707 measures the dissolved oxygen (DO) consumption during aerobic biodegradation in a closed bottle setup without head space. The degradation percentage is calculated as the ratio of DO consumption to the theoretical oxygen demand (ThOD).

Compared to some other methods, ISO 10707 has the top levels of simplicity and applicability.

The formula of the test substance and its purity may be known to calculate the ThOD. If the ThOD cannot be obtained, the chemical oxygen demand (COD) may be experimentally determined and used as an alternative.

Information on the toxicity of the test substance is helpful in determining the appropriate dosage so that the material does not inhibit bacteria at the concentration tested.

This method is capable of testing samples that are highly soluble, volatile, and/or adsorbing

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ISO 10707 Requirement and Applicability

Different testing methods are applicable for materials with different properties. Below is a summary of the applicability of ISO 10707. Please check our Method Selection Guide to select the most appropriate method for your materials. You can also find the applicability for many other methods on Aropha Resource Center

TestAnalytical methodSample info required *Poorly solubleVolatileAdsorbing
ISO 10707 (Closed bottle test)
Dissolved oxygen
ThOD or COD *

*"Sample info required" is the information needed to calculate the biodegradation percentages. This must be available for a selected method.
*"ThOD" can be easily calculated based on the formula of the sample. Try our Online ThOD Calculator.
*"COD" can be used as an alternative to ThOD if the formula of the material is unknown. We provide COD analysis at Aropha.

Aropha Resource Center

To learn more about different types of biodegradation tests, their applicability, biodegradation mechanisms, and many other information such as case studies, publications, and blogs, please check our Aropha Resource Center

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